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All authors contributed to critical revision toward and approved the final manuscript. Authors’ Affiliations (2) References Agurell S Halldin M Lindgren JE Ohlsson A Widman M Gillespie H Hollister L: Pharmacokinetics and metabolism of hemp oil vs olive oil delta-1-tetrahydrocannabinol and other cannabinoids with emphasis on man. Pharmacol Rev 1986 38:21-43.

The temperature was set to 230C unless specified otherwise. All experiments were carried out in a standard laboratory how to extract hash oil from weed fume hood under constant

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ventilation with an ambient room temperature of about 20C and a humidity of 40-60%. hemp oil elixinol Crystalline form CBD (preliminary experiments) or ethanolic solutions of CBD and THC (confirmatory and replication studies) were loaded onto the vaporiser filling chamber via a liquid pad – a removable disc made of tightly packed stainless steel wire mesh – as supplied by the manufacturer of the Volcano. For the confirmatory and replication studies the desired dose of cannabinoids (see Table

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1 ) was applied in aliquots of maximum 400?l in order to prevent overloading of the liquid pad which may result in leaking. After each application ethanol was evaporated using the Volcano vaporiser set at 100C for 30seconds. THC and CBD (with boiling points well over 160C) were not affected by this treatment and remained as pure compounds on the liquid cbd tincture san diego pad.