Cells were cultured in 75-cm2 culturing flasks cbd acronym medical in DMEM supplemented with 4 mM l-glutamine 100 units/ml penicillin 100 ?g/ml streptomycin 1% sodium pyruvate 1% nonessential amino Can You Put Hemp Seed Oil On Your Face acids and 10% heat-inactivated fetal bovine serum. Can You Put Hemp Seed Can You Put Hemp Seed Oil On Your Face Oil On Your Face for in vitro hemp seed oil treatment eczema studies cells were seeded in serum-free medium consisting of DMEM supplemented with 5 ?g/ml insulin 5 ?g/ml transferrin and 5 ng/ml sodium selenite in multiwell plates or Petri dishes according to experimental protocol. After a 24-h incubation the medium was removed and new culture medium containing the compounds to be tested was added. Analysis of Cell Viability.
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U87 and U373 respectively as compared with the control. Further inhibition in the MTT test was observed with 20 ?M (28 2% for U87 and 40 3.2% for U373 n = 12) 30 ?M (70 3.5% for U87 and 70 3.43 for U373 n = 12) and 40 ?M (96 10% for U87 and 94 10.34 for U373 n = 12) with IC50 values of 26.2 2.8 ?M in U87 cells and 24.1 2.16 ?M for U373. In a subsequent series of experiments we tested the effect of a single administration of CBD (using the mean IC50 concentration of 25 ?M) to the cells following their growth during a 4-day period. When the MTT test was Can You Put Hemp Seed Oil On Your Face performed daily for 4 days we found that the growth inhibition for both cell lines ( Fig. 1 A-C ) was still present during this period with a maximum effect seen at 4 days. Interestingly these results were positively correlated with the drop in cell number in a time-dependent manner ( Fig. 1 B-D ) as assessed by counting the cells by trypan blue exclusion.