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C ) but TRAIL levels did not change (data not shown). There was a reduction in pro-caspase-8 levels in the whole cell lysates ( Fig. 4C ) and the concurrent appearance of activated cleaved caspase-8 after treatment with CBD ( Fig. 4C ). We also observed the appearance of t-BID in the total cell lysates after treatment with similar CBD concentrations ( Fig. 4C ).
AKT is a survival molecule and its decreased phosphorylation precedes the induction of both autophagy and apoptosis ( 22 ). We observed decreased levels of phosphorylated AKT between 4 and 24 hours after treatment with CBD ( Fig:
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